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sion of SRB-1 in rat PCAs was evaluated by real-time PCR. Rat PCAs had been isolated and cleaned of luminal blood and total mRNA was isolated working with an RNA Mini Kit. Arteries from three 3 rats had been pooled per sample, and three samples had been utilized for real-time PCR. The mRNA was transcribed applying an iScript cDNA Synthesis Kit and 300 ng/ml IGFBP-3. B. Effects of intraluminal IGFB