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Human Prostate Cancer PC-3 Cells. PLoS 1 7: e41096. doi:ten.1371/journal.pone.0041096 Editor: Masuko Ushio-Fukai, University of Illinois at Chicago, United states of america of America Received April 9, 2011; Accepted June 21, 2012; Published July 20, 2012 Copyright: 2012 Lin et al. This really is an open-access short article distributed below the terms with the Inventive Commons Attribution Licen
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ne-array analyses showed that following IR, the expression of 95 genes was modified following two basic patterns. One of the most prominent pattern, shown by 81 from the genes, was a damage-like response. The genes exhibiting this pattern had been grouped into categories connected mainly with protein synthesis and secretion, indicating a reduction within the cell's ability to generate and secrete
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ys choice in ganciclovir, cells had been treated with trypsin/EDTA option, fixed in 2% paraformaldehyde, and permeabilized by suspension in PBS containing 0.1% Triton X100. 56105 cells have been mixed with 1 ml of mouse anti-human Cytokeratin 7 antibody . Secondary antibodies used were goat antimouse IgG-R-phycoerythrin antibody from Sigma. The samples were analyzed on a FACS Calibur flow cytomete
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