or samples, and it attenuated docetaxal-induced cell death. Herein, we showed that LEDGF expression was induced by LPA remedy in an LPA1/3-dependent manner. These benefits further support LPA being a crucial regulator in prostate cancer progression. Expression levels of ATX, an LPA-producing enzyme, are higher in androgen-independent prostate cancer cells. Additionally, clinical information reveal
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ith ChIP-qPCR primer sets. For nuclear extracts, the C2C12 cells have been homogenized in hypotonic buffer. The nuclei were incubated for 10 min on ice and then pelleted by centrifugation at eight,000 g, and resuspended in BC1000 buffer containing 0.1% NP40 to extract nuclear proteins. The samples had been then sonicated, centrifuged at 16,000 g, and dialyzed against BC200 containing 0.1% NP40. Th
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