idney Bone Metastasis activated flow cytometry analyses were performed on a FACScan flow cytometer. Immunofluorescence Cells were seeded into 24-well plate with cover slip for 48h followed by fixation with cold methanol for 10 min. After washing, cells were incubated with blocking solution containing 2% normal donkey serum, 1% bovine serum albumin, and 0.01% Triton X-100 in PBS for 30 min at room
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